Three phase partitioning of carbohydrate polymers: separation and purification of alginates
نویسندگان
چکیده
منابع مشابه
Three phase partitioning of carbohydrate polymers: separation and purification of alginates
Three phase partitioning, a technique described for protein purification, has been employed for precipitation and purification of three different commercial preparations of alginates. Three phase partitioning works by the addition of /-butanol to aqueous solution of the polymer containing 20-30% ammonium sulphate (w/v). Three phases formed are: upper /-butanol layer, interfacial polymer precipi...
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The technique of three-phase partitioning (TPP) was used to purify the green fluorescent protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert-butanol to precipitate proteins from their crude extracts. In the first round of TPP with 20% ammonium sulphate saturation at the ratio of crude to tert-butanol 1:1 (v/v), most of the GFP remains in the lower aqueous phase....
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ژورنال
عنوان ژورنال: Carbohydrate Polymers
سال: 2002
ISSN: 0144-8617
DOI: 10.1016/s0144-8617(01)00313-7